Home Protein'protein Interactions: Methods and Applications by Haian Fu (Editor) (ISBN: 9781588291202)

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Protein'protein Interactions: Methods and Applications Hardcover - 2004

Name: Protein - Protein Interactions: Methods and Applications (Methods in Molecular Biology) (Methods in Molecular Biology, 261)
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ISBN: 9781588291202

by Haian Fu (Editor)

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From the publisher

As the mysteries stored in our DNA have been more completely revealed, scientists have begun to face the extraordinary challenge of unraveling the int- cate network of protein-protein interactions established by that DNA fra- work. It is increasingly clear that proteins continuously interact with one another in a highly regulated fashion to determine cell fate, such as proliferation, diff- entiation, or death. These protein-protein interactions enable and exert str- gent control over DNA replication, RNA transcription, protein translation, macromolecular assembly and degradation, and signal transduction; essentially all cellular functions involve protein-protein interactions. Thus, protein-p- tein interactions are fundamental for normal physiology in all organisms. Alt- ation of critical protein-protein interactions is thought to be involved in the development of many diseases, such as neurodegenerative disorders, cancers, and infectious diseases. Therefore, examination of when and how protein-p- tein interactions occur and how they are controlled is essential for understa- ing diverse biological processes as well as for elucidating the molecular basis of diseases and identifying potential targets for therapeutic interventions. Over the years, many innovative biochemical, biophysical, genetic, and computational approaches have been developed to detect and analyze p- tein-protein interactions. This multitude of techniques is mandated by the diversity of physical and chemical properties of proteins and the sensitivity of protein-protein interactions to cellular conditions.

First line

Regulated interactions between proteins govern signaling pathways within and between cells.

From the rear cover

Proteins continuously interact with each other to determine cell fate. Consequently, an examination of just when such protein-protein interactions occur and how they are controlled is essential for understanding the molecular mechanism of biological processes, elucidating the molecular basis of diseases, and identifying potential targets for therapeutic interventions. In Protein-Protein Interactions: Methods and Applications, leading experts describe in detail their highly successful biochemical, biophysical, genetic, and computational techniques for studying these interactions. Their readily reproducible methods demonstrate how to identify protein interaction partners, qualitatively or quantitatively measure protein-protein interactions, monitor protein-protein interactions as they occur in living cells, and determine interaction interfaces. The techniques described utilize a variety of cutting-edge technologies, including surface plasmon resonance (SRP), fluorescence resonance energy transfer (FRET), fluorescence polarization (FP), isothermal titration calorimetry (ITC), circular dichroism (CD), protein fragment complementation assays (PCA), various two-hybrid systems, and proteomics- and bioinformatics-based approaches, such as the Scansite program for computational analysis. Each time-tested protocol includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Protein-Protein Interactions: Methods and Applications offers both beginning and experienced investigators a full range of the powerful tools needed for deciphering how proteins interact to form biological networks, as well as for unraveling protein-protein interactions in disease in the search for novel therapeutic targets.

Details

Title Protein'protein Interactions: Methods and Applications
Author Haian Fu (Editor)
Binding Hardcover
Edition 1st
Pages 532
Volumes 1
Language ENG
Publisher Humana
Date 2004-03-23
Illustrated Yes
ISBN 9781588291202 / 1588291200
Weight 1.98 lbs (0.90 kg)
Dimensions 9.28 x 6.22 x 1.22 in (23.57 x 15.80 x 3.10 cm)
Library of Congress subjects Protein-protein interactions, Protein Binding - physiology
Library of Congress Catalog Number 2003020640
Dewey Decimal Code 572.6